Botryosphaeria fujianensis
Botryosphaeria fujianensis Z.P. Dou, W. He & Y. Zhang, Mycosystema 40: 482.
Index Fungorum number: IF826938.
Saprobic on twigs of Tectona grandis L.F. Sexual morph: Ascomata 140–216 × 178–278 μm (x̅ = 182 × 211 μm, n = 20), well-developed, bursting through the bark, generally emerged from the host surface, scattered or gregarious, black, subglobose to obpyriform. Peridium 16–56 μm wide, composed of five strata; an outer stratum has black, thick-walled cells, and the middle and inner layers have black, thin-walled cells. Hamathecium comprising up to 3–5 μm wide, dense, cellular pseudoparaphyses, anastomosing above and between the asci. Asci 76–125 × 20–33 μm (n = 20), bitunicate, eight-spored, broadly clavate, apically rounded and with a visible ocular chamber. Ascospores 20–27 × 7–11 μm (x̅ = 24 × 10 μm, n = 50), initially rhomboid, hyaline, aseptate, elliptical to ovoid, thick-walled, smooth at maturity, apiculus at either end. Asexual morph: Not observed.
Culture characteristics: Ascospores germinating on PDA within 12 h. Colonies are fast growing on PDA, reaching 60 mm diam. after 7 d at 20–25 °C. Circular, white in first few days, becoming pale grey from the center after two weeks, and finally black after three weeks, felt-like, flattened, surface smooth.
Material examined: Thailand, Chiang Mai Province, Amphoe Mae Taeng, Tambon Pa Pae, 19°06′32.6″ N, 98°44′21.1″ E, on dead twigs of Tectona grandis (Lamiaceae), 8 August 2019, Na Wu, YW246 (MFLU 23-0012), living culture MFLUCC 23-0041.
Notes: The phylogenetic results showed that our newly obtained isolate clustered together with Botryosphaeria fujianensis, and we identified it as B. fujianensis. There was only the asexual morph provided when Chu et al. described this species, and we illustrate the sexual morph of B. fujianensis and reported it as the new record from Thailand in this study.
Figure 1. Botryosphaeria fujianensis (MFLU 23-0012). (a–c) Ascomata on host substrate. (d) Vertical section of ascomata. (e) Structure of the peridium. (f) Pseudoparaphyses. (g,h) Asci. (i,j) Ascospores. (k) Germinated ascospore. (l–n) Colonies on PDA, above (l,n) and below (m). Scale bars: (d) = 50 μm, (e) = 5 μm, (f–j) = 10 μm, (k) = 50 μm.
References:
Chu, R.T.; Dou, Z.P.; He, W.; Zhang. Y. Two novel species of Botryosphaeria causing stem canker of blueberries from China. Mycosystema, 2021, 40, 473–486. https://doi.org/10.13346/j.mycosystema.200333.
Wu, N.; Dissanayake, A.J.; Du, H.-Z.; Liu, J.-K. Morpho-Phylogenetic Evidence Reveals Novel Species and New Records of Botryosphaeriaceae in China and Thailand. J. Fungi 2023, 9, 1051. https://doi.org/10.3390/jof9111051
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