Diplodia mutila
Diplodia mutila Fries in Montagne, Ann. Sci. nat., se ́r. 21: 302.
Index Fungorum number: IF201741; Facesoffungi number: FoF00147.
Saprobic on dead twigs of Prunus persica L. Sexual morph: Not observed. Asexual morph: Conidiomata 213–306 × 247–313 μm (x̅ = 266 × 282 μm, n = 20), semi-immersed or immersed in the substrate, dark brown to black, solitary, globose to ovoid, centrally ostiolate. Ostiole 28–45 μm diam., centrally located, short papillate. Peridium up to 53–78 μm wide, 5–7 layers, consisting of brown and small-celled textura angularis. Conidiophores are reduced to conidiogenous cells. Conidiogenous cells 8–14 × 3–7 μm (x̅ = 9 × 4 μm, n = 20), hyaline, cylindrical, discrete, smooth-walled, slightly swollen at the base, forming a single conidium at the tip. Conidia 23–29 × 12–15 μm (x̅ = 27 × 14 μm, n = 50), hyaline, aseptate, externally smooth, internally verruculose, thick-walled, oblong to ovoid, straight, both ends broadly rounded.
Culture characteristics: Conidia germinating on PDA within 24 h. Colonies are fast growing at 20–25 °C, becoming ash-grey on the surface after 7 days and finally black after two weeks, felt-like, dense, convex with the papillate surface, aerial.
Material examined: China, Sichuan Province, Chengdu City, High Tech West Zone, Xi Yuan Avenue, University of Electronic Science and Technology of China campus, 30°45’9’’ N, 103°55’31’’ E, on dead twigs of Prunus persica L., 22 November 2020, H.Z. Du, YW290 (GZAAS 23-0582), living culture GZCC 23-0578.
Notes: We identified our isolate as Diplodia mutila based on morphology and phylogeny. This is the first record of Di. mutila found on Prunus persica in China.
Figure 1. Diplodia mutila (GZAAS 23-0582). (a–c) Conidiomata on host substrate. (d,e) Vertical section of conidiomata. (f) Section of peridium. (g–i) Conidiogenous cells and developing conidia. (j–m) Conidia. (n,o) Colonies on PDA, above (n) and below (o). Scale bars: (d–f) = 20 μm, (g–m) = 10 μm.
References:
Montagne, J.F.C. Notice sur les plantes cryptogames récemment découvertes en France contenant aussi l’indication précis des localités de quelques espèces les plus rares de la flore française. Ann. Des Sci. Nat. Bot. 1834, 2, 295–307.
Wu, N.; Dissanayake, A.J.; Du, H.-Z.; Liu, J.-K. Morpho-Phylogenetic Evidence Reveals Novel Species and New Records of Botryosphaeriaceae in China and Thailand. J. Fungi 2023, 9, 1051. https://doi.org/10.3390/jof9111051
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