Lasiodiplodia avicenniae
Lasiodiplodia avicenniae J.A. Osorio, Jol. Roux & Z.W. de Beer, Fungal Biology 121: 374 2016.
Index Fungorum: IF 812010; MycoBank: MB 812010.
Sexual morph: not observed. Asexual morph: Conidiomata pycnidial, solitary, immersed, dark brown to black, thick-walled textura angularis (238–)317–485(–560) μm wide, conidia oozing after 3 weeks. Paraphyses up to 170 μm long, 2–4 μm wide, hyaline, branched, septate. Conidiophores absent or reduced to a supporting cell. Conidiogenous cells (6–)9–11(–15) × (3–)3.5–4.2(– 6) μm, holoblastic, discrete, cylindrical, hyaline, smooth, proliferating at the same level giving rise, or proliferating percurrently to form one to two annellations. Conidia (19–) 24–26(-30) × (9–)12–12.5(–15) μm, hyaline, aseptate, obpyriform or ellipsoid, sometimes constricted in the central, contents granular, smooth, thick-walled, becoming brown and septate when mature.
Culture characteristics: Colonies reaching 86 mm diam. on MEA after 4 d in the dark at 25 °C. Cardinal temperatures for growth: min 10 °C, max < 35 °C, opt 20−25 °C.
Representative isolate: South Africa, KwaZulu-Natal province, Beachwood and Isipingo, from asymptomatic branches of Avicennia marina. J.A Osorio & Jol. Roux, holotype PREM 61249, CBS 139670 (ex-type culture).
Hosts: Avicennia marina (Osorio et al. 2016).
Known distribution: Beachwood and Isipingo in Durban (KwaZulu-Natal Province, South Africa).
GenBank Numbers: ITS KP860835, tef1-α KP860680, tub2 KP860758, rpb2 KU587878
Notes: The paraphyses of L. avicenniae are septate, and significantly longer (170 μm) than those of L. pseudotheobromae (58 μm). Conidial shape is variable in L. avicenniae, obpyriform, ellipsoid and sometimes constricted in the middle and smaller (24–26 × 12–12.5 μm) compared with those of L. pseudotheobromae, which are ellipsoid and widest at the middle (23.5–32 × 14–18 μm).
References:
Osorio JA, Crous CJ, De Beer ZW, Wingfield MJ, & Roux J (2017). Endophytic Botryosphaeriaceae, including five new species, associated with mangrove trees in South Africa. Fungal biology 121: 361–393.