Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia chonburiensis

Lasiodiplodia chonburiensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [54] (2018)

 

Index Fungorum number: IF554472

Facesoffungi number: FoF04564

Etymology: named after Chonburi Province, where the fungus was first discovered.

Holotype: MFLU 16-1877

 

Saprobic on dead leaves of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 210–250 ´ 270–300 μm (   = 236 ´ 287 μm, n = 5), scattered to gregarious, immersed, conspicuous on host surface, dark brown, solitary, uniloculate, globose to subglobose, ostiole without papilla. Pycnidial wall 20–51 μm, composed of several layers of thick-walled, hyaline to dark brown cells of textura angularis. Conidiogenous cells 9–13 ´ 3–5 μm ( = 11 ´ 4 μm, n = 20), annellidic, cylindrical, thick- walled, smooth. Conidia 15–30 ´ 10–15 μm ( = 23 ´ 12 μm, n = 30), subglobose to oval, aseptate, hyaline to subhyaline with age, guttulate, without longitu- dinal striations and mucilaginous sheath.

Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA, white at first, becoming black with age, circular, with entire edge, raised, velvety, black in reverse, with smooth margin.

Material examined: THAILAND, Chonburi Province, Bang Lamung District, on dead leaf of Pandanus sp., 18 July 2016, W. Jaidee PTY02 (MFLU 16-1877, holotype; HKAS 96271, isotype); ex-type living culture, MFLUCC 16-0376 = KUMCC 17-0299.

GenBank numbers: LSU: MH260299; ITS: MH275066; TEF1: MH412773; TUB2: MH412742.

Notes: Lasiodiplodia chonburiensis clusters with L. caatinguensis I.B.L. Cout., F.C. Freire, C.S. Lima & J.E. Cardoso in phylogenetic analyses. Lasiodiplodia caatinguensis has ovoid to ellipsoids conidia 18.15 ´ 11.78 μm (Coutinho et al. 2017), while L. chonburiensis has subglobose to oval conidia 23 ´ 12 lm. Therefore, L. chonburiensis is intro- duced as a new species based on morphology and phy- logeny. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 16-0376 is Lasiodiplodia sp. with 96% identity to the strain CMW40968 (KP872323), while the closest matches with the TEF1 sequence were with 100% identical L. theobro- mae strain CBS190.73 (EF622048) and closest matches with the TUB2 sequence were with 99% identical L. theobromae strain L10 (KR260829).

Fig. 1: Lasiodiplodia chonburiensis (MFLU 16-1877, holotype). a Appearance of conidiomata on host substrate. b Section of conidioma.
c Section of pycnidial wall. d, e Conidiogenous cell producing conidia. f–h Conidia.
i Germinating conidium. Scale bars: a = 200 μm, b = 100 μm, c = 20 μm, d, e = 5 μm, f–
i = 10 μm.

References:

Coutinho IBL, Freire FCO, Lima CS, Lima JS, Gonc ̧alves FJT, Machado AR, Silva AMS, Cardoso JE (2017) Diversity of genus Lasiodiplodia associated with perennial tropical fruit plants in northeastern Brazil. Plant Pathol 66:90–104.

 

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