Lasiodiplodia mahajangana
Lasiodiplodia mahajangana Begoude, Jol. Roux & Slippers, Mycol. Progr. 9: 110 (2010) Figure 11.
Index Fungorum number: IF514012.
Saprobic on dead seeds of Dipterocarpus retusus L. Sexual morph: Not observed. Asexual morph: Conidiomata 127–164 × 133–192 μm (x̅ = 148 × 160 μm, n = 20), solitary or compound, superficial or immersed, unilocular or multilocular, globose to subglobose, thick-walled, glabrous, ostiolate. Ostiole 26–33 μm diam., single, long, cylindrical to subcylindrical, eccentric. Peridium up to 14–23 μm wide, consisting of brown and small-celled textura angularis. Paraphyses 2–4 μm wide, hyaline, cylindrical, aseptate, not branched, rounded at apex. Conidiophores are reduced to conidiogenous cells. Conidiogenous cells 6–13 × 4–5 μm (x̅ = 8 × 5 μm, n = 20), hyaline, cylindrical, proliferating percurrently to form a periclinal thickening. Conidia 24–31 × 14–18 μm (x̅ = 27 × 16 μm, n = 50), initially aseptate, hyaline, ellipsoid to ovoid, thick-walled with granular content, rounded at apex, occasionally truncate at the base, one septate at maturation, without longitudinal striations or mucilaginous sheath.
Culture characteristics: Conidia germinating on PDA within 24 h. Colonies are fast growing on PDA at 20–25 °C, becoming ash-grey on the surface after 7 days, with the reverse side of the colonies pale grey to grey, and finally black after two weeks, felt-like, sparse, aerial, surface smooth with crenate edge, filamentous.
Material examined: Thailand, Chiang Mai Province, Amphoe Mae Taeng, Tambon Cho Lae, 19°08’01.3’’ N, 99°00’29.4’’ E, on dead seeds of Dipterocarpus retusus L., 10 August 2019, Na Wu, YW151 (MFLU 23-0006), living culture MFLUCC 23-0059.
Notes: In the phylogenetic tree, an isolate obtained in this study (MFLUCC 23-0059) grouped with Lasiodiplodia mahajangana (Figure 1) (ML/MP/BI = 94/95/1.0). Our sample is morphologically similar to L. mahajangana as of the report by Begoude et al. (2010), having hyaline, aseptate, ellipsoid to ovoid, thick-walled conidia, which becomes one septate after maturation. We identified our collection as L. mahajangana based on morphology and phylogeny.
Figure 1. Lasiodiplodia mahajangana (MFLU 23-0006). (a–c) Conidiomata on host substrate. (d) Vertical section of conidiomata. (e) Section of peridium. (f) Paraphyses. (g–j) Conidiogenous cells and developing conidia. (k–n) Conidia. (o) Germinated conidium. (p–r) Colonies on PDA, above (p,r) and below (q). Scale bars: (b) = 100 μm, (c) = 20 μm, (d) = 50 μm, (e) = 20 μm, (f–o) = 10 μm.
References:
Begoude, B.A.D.; Slippers, B.; Wingfield, M.J.; Roux, J. Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar. Mycol. Prog. 2010, 9, 101–123.
Wu, N.; Dissanayake, A.J.; Du, H.-Z.; Liu, J.-K. Morpho-Phylogenetic Evidence Reveals Novel Species and New Records of Botryosphaeriaceae in China and Thailand. J. Fungi 2023, 9, 1051. https://doi.org/10.3390/jof9111051
Recent Notes
Economic and ecological significance of the families in Botryosphaerialesbotryosphaeriales.org Note 1
Recent Genus
PileosporaSeptorioides
Pileospora
Recent Species
Dothiorella macadamiaeDiplodia peckiana
Barriopsis caryotae