Lasiodiplodia marypalme
Lasiodiplodia marypalme M.S.B. Netto, M.W. Marques, A.J.L. Phillips & M.P.S. Câmara [as 'marypalme'], in Netto, Assunção, Lima, Marques, Lima, Monteiro, de Queiroz Balbino, Michereff, Phillips & Câmara, Fungal Diversity 67: 136 (2014)
MycoBank: MB807526
Etymology: named in honor of Mary E. Palm, United States Department of Agriculture - APHIS, for her contribution to mycology.
Ascomata not seen. Conidiomata: stromatic, pycnidial, produced on pine needles on WA within 2–4 weeks, superfi- cial, dark brown to black, covered with dense mycelium, mostly uniloculate, solitary, globose, thick-walled, non- papillate with a central ostiole. Paraphyses: hyaline, cylindri- cal, aseptate, rounded at apex. Conidiophores: absent. Conidiogenous cells: holoblastic, discrete, hyaline, smooth, thin-walled, cylindrical. Conidia: initially hyaline, aseptate, ellipsoid to ovoid, with granular content, rounded at apex, base mostly truncate, wall <2 μm, becoming pigmented, verruculose, ellipsoid to ovoid, 1-septate with longitudinal striations, 19.1– 28.5 × 10–15.3 μm.
Culture characteristics: Colonies with abundant aerial mycelia reaching to the lid of Petri plate, aerial mycelia becoming mouse grey (13””’i) to pale mouse grey (17””’i), at the surface and grey olivaceous (21””b) to olivaceous black (27””m) at the reverse. Colonies reaching 39 mm on MEA after 2 days in the dark at 25 °C. Optimum temperature for mycelial growth: 32.8 ± 2.12 °C, covering the surface of 90 mm diam. Petri dishes within 3 days on MEA in the dark
Substrate: Carica papaya
Known Distribution: Brazil (Pernambuco, Rio Grande do Norte).
Holotype: Brazil, Pernambuco, Goiana, Farm Ubu 92 (34° 56′ 16.5′′, 7° 42′ 32.6′′), on Carica papaya fruit, 2007, coll. J.H.A. Monteiro, holotype URM (85579) a dry culture on pine needles, ex-holotype living culture CMM 2275 = URM 7117.
Specimen examined: Brazil, Pernambuco, Goiana, Farm Ubu 92 (34° 56′ 16.5′′, 07° 42′ 32.6′′), on Carica papaya fruit, 2007, coll. J.H.A. Monteiro (CMM 2272). Brazil, Pernambuco, Goiana, Farm Ubu 92 (34° 56′ 16.5′′, 07° 42′ 32.6′′), on Carica papaya fruit, 2007, coll. J.H.A. Monteiro (CMM 2271). Brazil, Pernambuco, Goiana, Farm Ubu 92 (34° 56′ 16.5′′, 07° 42′ 32.6′′), on Carica papaya fruit, 2007, coll. J.H.A. Monteiro (CMM 2274). Brazil, Rio Grande do Norte, Parnamirim, Farm Arco Verde (35° 16′ 36.4′′, 05° 56′ 30.2′′), on Carica papaya fruit, 2006, coll. J.H.A. Monteiro (CMM 2173). Brazil, Rio Grande do Norte, São José do Mipibú, Farm Vale do Lírio (35° 17′ 48.8′′, 06° 00′ 50.2′′), Carica papaya fruit, 2007, coll. J.H.A. Monteiro (CMM 2298). Brazil, Rio Grande do Norte, São José do Mipibú, Farm Vale do Lírio (37° 22′ 06.4′′, 04° 54′ 13.4′′), on Carica papaya fruit, 2007, coll. J.H.A. Monteiro (CMM 2289).
Notes: Phylogenetically Lasiodiplodia marypalme is closely related to L. pseudotheobromae and L. citricola, but conidia of L. citricola, 22.5–26.6 × 13.6–17.2 μm and L. pseudotheobromae L. pseudotheobromae 23.5–32 × 14– 18, are longer and wider than those of L. marypalme, 18.0– 24.4 × 9.8–15.3 μm. Lasiodiplodia marypalme differs from its closest phylogenetic neighbor, L. pseudotheobromae and L. citricola, by unique fixed alleles in two loci based on alignments of the separate loci deposited in TreeBase as study S14682: ITS positions 12 (A), 68 (C), 86 (T) and 409 (C); EF- 1α positions 19(C) and 43 (T), 51 (T), 128 (GAP), 166 (G), 185 (A), 220 (G), 221 (C) and 229 (T).
Figure 1: Lasiodiplodia marypalme holotype. a Conidia developing on conidiogenous cells between paraphyses. b Hyaline immature conidia and mature conidia. c–d Mature conidia in two different focal planes to show the longitudinal striations. Scale bars: a–d=10 μm.