Lasiodiplodia mediterranea Linald., Deidda & A.J.L. Phillips, in Linaldeddu, Deidda, Scanu, Franceschini, Serra, Berraf-Tebbal, Zouaoui, Boutiti, Jama & Phillips, Fungal Diversit.
MycoBank: MB 808356
Etymology: Named for the Mediterranean region where this fungus was isolated for the first time.
Sexual state: Not seen. Asexual state: Conidiomata pycnidial formed on poplar twigs in culture within 2–3 weeks, uniloculate, dark brown to black, immersed in the host becoming erumpent when mature. Paraphyses hyaline, cylindrical, septate, sometimes branched, ends rounded, measuring 87 ± 19.9 × 2.7 ± 0.6 μm (mean ± S.D., n = 20). Conidiogenous cells 13.6 ± 2.2 × 3.7 ± 1 μm (mean ± S.D., n = 20), hyaline, smooth, cylindrical, sometimes slightly swollen at the base, holoblastic forming conidia at their tips, proliferating internally giving rise to periclinal thickenings. Conidia subcylindrical to elliptical, apex and base rounded, typically widest at the middle, thick-walled, initially hyaline and aseptate and remaining so for a long time, becoming one or two-septate and dark brown with age, with longitudinal striations (26.3–)30.6(−37) × (13.5–)16.1(−18) μm, 95 % confi- dence limits = 30–31.1 × 15.9–16.3 μm (mean ± S.D. = 30.6 ± 2.8 × 16.1 ± 0.9 μm, l/w ratio = 1.9 ± 0.2).
Cultural characteristics: Colonies on PDA grew rapidly, reaching 90 mm in diameter before 7 days at 25 °C, the mycelium was moderately aerial, surface white at first and later turned pale to dark grey from the centre to the margin and greyish to dark in reverse. Isolates growing at 35 °C produced a diffusible pink pigment within 3 days (Fig. 4).
Cardinal temperatures for growth: Minimum <10 °C, maximum <40 °C and optimum 25–30 °C.
Habitat: On trunk and branches of Vitis vinifera, Quercus ilex and Citrus×sinensis.
Known distribution: Italy and Algeria.
Specimens examined: ITALY, Bortigiadas, isolated from a branch canker of Quercus ilex, June 2004, Benedetto T. Linaldeddu, HOLOTYPE LISE 96303, a dried culture sporu- lating on Quercus ilex, culture ex-holotype CBS 137783= BL1. ITALY, Badesi, isolated from a brown stripe under the bark on Vitis vinifera, 11 February 2010, Salvatorica Serra (culture BL101 = CBS 137784). Other isolates examined are listed in Table 2.
Notes: Lasiodiplodia mediterranea is phylogenetically closely related to L. pseudotheobromae, but can be distinguished based on the shape and dimensions of conidia and paraphyses.