Lasiodiplodia missouriana
Lasiodiplodia missouriana Úrbez-Torr., Peduto & Gubler, in Úrbez-Torres, Peduto, Striegler, Urrea-Romero, Rupe, Cartwright & Gubler, Fungal Diversity 55(1): 181 (2012)
Mycobank: 519954
Etymology: Named after the State of Missouri where it was first found.
Pycnidia, gregariis, niger, mycelio fusco circumdata et stromate inclusa, globosis, profundus-murus, uniloculare, ostiole erumpente, usque ad 320 μm diameter. Paraphyses hyalinae, cylindricae, non-septatis. Cellulae conidiogenae holoblasticae, hyalinae, teres, cylindricae. Conidia primo hyalinis, unicellularia, ellipsoidea vel obovoidea, profundusmurus (murus 1–2 μm), contento granulari, demum atrer brunneis, uniseptatis, in matures strias verticalis visas, (16.1-) 17.4-19.6(-21) × (8.1-)8.9-10.6(-11.8) μm (statura media ± 18.5 × 9.8) (longitudo/latitudo 1.89). Mediocriter aerious mycelio, primo albus, demum viridi-olivaceus ad viride niger.
Pycnidia, superficial, stromatic, formed on PDA within 2–3 weeks, black, covered with moderate mycelium, up to 320 μm wide, globose to ovoid, thick-walled, unilocular, with a central ostiole, often oozing conidia. Paraphyses hyaline, cylindrical, aseptate, not branched, round at apex, up to 55 μm long, 2–3 μm wide. Conidiogenous cells holoblastic, hyaline, smooth, cylindrical. Conidia produced in culture initially hyaline, unicellular, ellipsoid to ovoid, thick-walled (wall 1–2 μm), granular content, becoming dark brown, one-septate, with longitudinal striations while still inside the pycnidia, (16.1-)17.4-19.6(-21) × (8.1-)8.9- 10.6(-11.8) μm (av. ±18.5 × 9.8) (l/w ratio = 1.89 ± 0.3).
Cultural characteristics: Moderate aerial mycelium on PDA. Aerial mycelia/colonies on PDA initially white becoming pale olivaceous grey (23””’f) within 7 days and turning iron grey (23””’k) to greenish black (24””’m) within 28 days at the surface. Colony dark slate blue (49”’i) at the reverse after 28 days. Colonies reaching 90 mm diameter on PDA after 48 h in the dark at 25°C. Cardinal temperatures for growth between 10 and 35°C. Optimum temperature for growth 25–30°C.
Habitat: interspecific hybrid grape Catawba (Vitis labrusca x Vitis vinifera hybrid (Vitis sp. x Vitis labruscana))
Known distribution: state of Missouri, USA
Material examined: USA, Saint James, Missouri, R. K. Striegler and G. M. Leavitt, Catawba (UCD2193MO; holotype, culture ex-type CBS128311); USA, Saint James, Missouri, R. K. Striegler and G. M. Leavitt, Catawba (UCD2199MO; CBS128312).
Figures 1: Lasiodiplodia missouriana CBS128311. 17, 7-days-old colony on PDA. 18, 28-days-old colony on PDA. 19, conidiomata on PDA culture showing oozing of conidia. 20, cross section of conidiomata showing individual pycnidium. Arrow shows cirrus of conidia oozing from one pycnidium. 21, conidia developing on conidiogenous cells. 22, paraphyses. 23, hyaline conidia. 24, pigmented conidia in two different focal planes to show the longitudinal striations. 25, hyaline and pigmented conidia. Scale bars: 19 and 20=1000 μm; 21 and 22=10 μm; 23=20 μm; 24=10 μm; 25=5 μm.