Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia pandanicola

Lasiodiplodia pandanicola Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [58] (2018)

 

Index Fungorum number: IF554473

Facesoffungi number: FoF04566

Etymology: named after the host genus, Pandanus

Holotype: MFLU 18-0011


 

Saprobic on dead root of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 185–210 ´ 187–240 μm (=198 ´ 211 μm, n=5), immersed to erumpent through host, visible as black, subglobose to ovoid, solitary, scattered or gregarious, ostiole. Pycnidial wall 20–42 μm, composed of several layers of textura angularis, dark brown. Conidiophores micronematous, reduced to conidiogenous cell. Conidiogenous cells 4–6.5 ´ 5–7 μm (=596 μm, n=5), phialidic, integrated, cylindrical or cylindric-clavate or irregular swollen cell, hyaline, smooth. Conidia 14– 38 ´ 9–22 μm (= 27 ´ 14 μm, n = 30), ellipsoidal to obovate, initially hyaline and aseptate, later becoming brown and 1-septate, thick and rough-walled, guttulate, with longitudinal striations.

Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA, initially white–grey and later become black, with dense mycelium, circular, with entire edge, raised, velvety, black in reverse, with smooth margin. Sporulating in culture after 2 months, producing conidia similar in shape to those recorded on natural dead leaves.

Material examined: THAILAND, Phatthalung Province, Mueang Phatthalung District, on dead leaves of Pandanus sp., 14 June 2015, B. Thongbai SF15-009 (MFLU 18-0011, holotype); ex-type living culture, MFLUCC 16-0265 = KUMCC 16-0158.

GenBank numbers: LSU: MH260301; ITS: MH275068; TEF1: MH412774; TUB2: MH412744.

Notes: Phylogenetic analyses indicated Lasiodiplodia pandanicola is well-separated with other Lasiodiplodia spp.. Lasiodiplodia parva A.J.L. Phillips, A. Alves & Crous share similar conidia to L. pandanicola. However, L. parva has coni- dia 16–23.5 ´ 10.5–13 μm, ovoid (Alves et al. 2008), while L. pandanicola has conidia 14–38 ´ 9–22 μm and ellipsoidal to obovate. Therefore, we propose L. pan- danicola as a new species. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 16-0265 is L. theobromae with 100% identity to the strain Lt-A2 (KX270362), while the closest mat- ches with the TEF1 sequence were with 100% identical L. theobromae strain IRNHM-KB64-2 (KU737511) and closest matches with the TUB2 sequence were with 95% identical Botryosphaeria quercuum strain CBS177.89 (DQ026404).

Fig. 1: Lasiodiplodia pandanicola (MFLU 18-0011, holotype). a Appearance of conidiomata on host substrate. b Section of conidioma.
c Section of pycnidial wall.
d Conidiogenous cell producing conidia. e–g Conidia.
h Conidium with longitudinal striations. i Germinating conidium. j, k Colony on MEA from above and below. l, m Conidia formed in culture. Scale bars: a = 200 μm, b = 100 μm, c, d, l, m = 20 μm, e–i = 5 μm.

References:

Alves A, Crous PW, Correia A, Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Divers 28:1–13

 

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