Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia viticola

Lasiodiplodia viticola Úrbez-Torr., Peduto & Gubler, in Úrbez-Torres, Peduto, Striegler, Urrea-Romero, Rupe, Cartwright & Gubler, Fungal Diversity 55(1): 183 (2012)

Mycobank: 519955


Pycnidia, sparsis, solitaria, carbonaceis, mycelio fusco circumdata, globosa, profundus-murus, uniloculare, ostiole erumpente, usque ad 900 μm diameter. Paraphyses hyalinae, cylindricae, non-septatis. Cellulae conidiogenae holoblasticae, hyalinae, contento granulari, cylindricae. Conidia primo hyalinis, unicellularia, ellipsoidea vel obovoidea, profundus-murus (murus 1–2 μm), contento granulari, demum brunneis, uniseptatis, in matures strias verticals visas, (16.8-)18.2-20.5(-22.9) × (7.9-)8.8-10.1(-10.7) μm (statura media ± 19.5 × 9.5) (longitudo/latitudo = 2.05). Abundantia aerious mycelio, primo subolivaceo-griseus, demum viridi- olivaceus ad viride niger.

Pycnidia, solitary, formed on PDA within 3–4 weeks, black, covered with moderate mycelium, up to 900 μm wide, globose to ovoid, thick-walled, unilocular, with a central ostiole, often oozing conidia. Paraphyses hyaline, cylindrical, aseptate, not branched, round at apex, up to 60 μm long, 2–3 μm wide. Conidiogenous cells holoblas- tic, hyaline, smooth, cylindrical. Conidia produced in culture initially hyaline, unicellular, ellipsoidal, base rounded or truncate, thick-walled (wall 1–2 μm), granular content, becoming dark brown, one-septate, with longitudinal stria- tions while still inside the pycnidia, (16.8-)18.2-20.5(-22.9)× (7.9-)8.8-10.1(-10.7) μm (av. ± 19.5 × 9.5) (l/w ratio = 2.05 ± 0.2).

Cultural characteristics: Dense aerial mycelium on PDA. Aerial mycelia/colonies on PDA initially white becoming pale olive-buff (21”’f) within 7 days and turning iron grey (23””’k) to greenish black (24””’m) within 28 days on the surface. Colony dark slate blue (49”’i) at the reverse after 28 days. Colonies reaching 90 mm diameter on PDA after 48 h in the dark at 25°C. Cardinal temperatures for growth between 10 and 35°C. Optimum temperature for growth 25–30°C.

Etymology: Name after the host from which it was first isolated, namely Vitis vinifera.

Habitat: interspecific hybrid grape Vignoles (Ravat 51). Known distribution: states of Arkansas and Missouri, USA Material examined: USA, Altus, Arkansas, R. D. Cartwright and W. D. Gubler, Vignoles (UCD2553AR; holotype, culture ex-type CBS128313); USA, Augusta, Missouri, K. Striegler & W. D. Gubler, Chardonel (UCD2604; CBS128315).