Phyllosticta hypoglossi (Mont.) Allesch., Rabenh. Krypt.-Fl., Edn 2 (Leipzig) 1(6): 163. 1898.
Index Fungorum: IF446040; Facesoffungi: FoFxxxxx; MycoBank: MB446040.
Pathogenic on cladodes of Ruscus hypoglossum. Sexual morph: Unknown. Asexual morph: Conidiomata (on PNA) up to 200 μm diam., pycnidial, solitary, black, erumpent, globose, exuding colourless conidial masses. Peridium up to 30 μm thick, composed of several layers of textura angularis, inner wall of hyaline textura angularis. Ostiole up to 15 μm diam., central. Conidiophores 15–25 × 4–5 μm, subcylindrical to ampulliform, reduced to conidiogenous cells, or with 1–2 supporting cells, that can be branched at the base. Conidiogenous cells 10–15 × 3–5 μm, terminal, subcylindrical, hyaline, smooth, coated in a mucoid layer, proliferating several times percurrently near apex. Conidia (10–)11–12(–14) × (9–)10(–11) μm, solitary, hyaline, aseptate, thin and smooth walled, granular, or with a single large central guttule, broadly ellipsoid to obovoid or globose, tapering towards a narrow truncate base, 3–4 μm diam., enclosed in a thin, mucoid sheath, 1–3 μm thick, mostly not persistent, and bearing a hyaline, apical mucoid appendage, (8–)10–12(–15) × 1.5(–2) μm, flexible, unbranched, tapering towards an acute tip.
Culture characteristics: Colonies flat, spreading with sparse aerial mycelium and feathery, lobate margins, reaching 25 mm diam. on MEA, 30 mm diam. on PDA and 35 mm diam. on OA after 2 wk at 25 ºC. On OA centre olivaceous-grey, outer zone with diffuse pale yellow pigment in agar. On PDA surface olivaceous-grey, reverse iron-grey. On MEA surface iron-grey in centre, pale grey-olivaceous in outer region, iron-grey in reverse.
Representative isolate: France, near Marseille, on cladodes of Ruscus hypoglossum, 1845, J.L.M. Castagne, (type not found, presumably missing). Italy, Prov. Napoli, Cratere degli Astroni, on dead cladodes of Ruscus aculeatus, May 1992, W. Gams, neotype CBS H-5331, CBS 434.92 = MBT176248 (ex-neotype culture).
Host: Ruscus hypoglossum (Wikee et al. 2013).
Known distribution: France (Wikee et al. 2013).
GenBank Numbers: ITS - FJ538367, LSU - KF206299, TEF - FJ538425, ACT - FJ538483, GPDH - JF343695.
Notes: Considering the number of specimens and cultures in the CBS culture collection, P. hypoglossi is a common European species on cladodes of Ruscus hypoglossum.
Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EHC, Hyde KD, Crous PW (2013) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Studies in Mycology 76: 1–29.