Pseudofusicoccum calophylli
Pseudofusicoccum calophylli Jayasiri, E.B.G. Jones & K.D. Hyde, in Jayasiri, Hyde, Jones, McKenzie, Jeewon, Phillips, Bhat, Wanasinghe, Liu, Lu, Kang, Xu & Karunarathna, Mycosphere 10(1): 151 (2019)
Index Fungorum number: IF555584
Facesoffungi number: FoF05299
Holotype: MFLU 18–2153
Etymology: Referring to the host genus on which the fungus was collected, Calophyllum
(Calophyllaceae)
Saprobic on Calophyllum inophyllum. Asexual morph: Undetermined. Asexual morph:
Coelomycetous. Conidiomata 140–160 μm high × 133–197 μm diam. ( x = 144 × 160 μm; n = 10), semi-immersed, solitary, globose to subglobose, papillate, covered by host epidermal tissues, lack of ostiole. Conidiomata wall 30–50 μm wide (x = 47 μm; n = 20), outer pale brown textura angularis cell layers, inner hyaline textura angularis cell layer, embedded within plant tissues. Conidiogenous cells 10–14 × 3–5 μm (x = 13 × 4 μm; n = 20), phialidic, ovate to cylindrical, smooth, hyaline. Conidia 14–17 × 4–5 μm (x = 16 × 4.5 μm; n = 30), hyaline, ellipsoid, occasionally slightly bent or irregularly shaped, apices rounded, smooth with fine granular content, unicellular, thin-walled.
Culture characters: Conidia germinated on MEA within 24 hr. Germ tubes produced at one end or both ends of conidia. Colonies growing on MEA, reaching 35–40 mm diam. after 2 weeks at 18°C. Colonies fluffy, initially white to amber at the centre, olivaceous at the edges, becoming white to olivaceous with age.
Material examined: THAILAND, Krabi Province, Mueang Krabi District (8 ̊ 2' 27" N, 98 ̊ 49' 5" E), decaying fruit pericarp of Calophyllum inophyllum (Calophyllaceae), 31 August 2018, S.C. Jayasiri, C 346 (MFLU 18–2153, holotype; KUN-HKAS102429, isotype), ex-type living culture MFLUCC 17–2533, KUMCC 18–0282.
GenBank numbers: ITS: MK347764, tef1: MK340877, rpb2: MK434879, tub2: MK412885
Notes: Pseudofusicoccum calophylli clusters with two strains of P. violaceum. Pseudofusicoccum violaceum is characterized by bacilliform conidia with a mucilaginous sheath and larger spores compared to P. calophylli (33 × 9.5 vs. 16 × 4.5 μm) (Mehl et al. 2011). However, Pseudofusicoccum calophylli has bacilliform conidia in the immature stage but these later become irregular in shape, without a mucilaginous sheath (Fig. 129). A comparison of the ITS and tef1 nucleotides of these two strains reveals 5 (0.8%) and 5 (1.6%) nucleotide differences, which indicates that they are distinct taxa (Jeewon & Hyde 2016).
Figure 1. Pseudofusicoccum calophylli (MFLU 18–2153, holotype). a Host fruit. b Conidiomata on host surface. c Section through conidioma. d–f Conidiogenus cells. g–j Conidia. k Germinated conidia. Scale bars: a = 1 cm, b = 500 μm, c = 50 μm, d–k = 10 μm.